Kassambara A, Schoenhals M, Moreaux J, et al. Inhibition of DEPDC1A Delays Growth and Induces Mature Plasma Cell Markers. PLoS One. 2013. Download the PDF
Summary of the Study
High-throughput microarray profiling has enabled the identification of genes associated with poor prognosis in multiple myeloma (MM). Among these, DEPDC1A, a gene encoding a protein with a Disheveled, EGL-10, and Pleckstrin (DEP) domain, is correlated with shorter patient survival.
This study investigates the biological role of DEPDC1A using lentiviral shRNA in human myeloma cell lines. Key findings include:
- DEPDC1A knockdown delayed cell growth by causing G2 cell cycle arrest, increasing p53 phosphorylation, and p21 accumulation
- It induced mature plasma cell markers, such as CXCR4, IL6-R, and CD38
- These results suggest that DEPDC1A contributes to maintaining plasmablast features in malignant plasma cells
DEPDC1A may therefore be a potential therapeutic target and a bad prognostic marker in MM.
In this study, Alboukadel Kassambara was the first author, leading the experimental design, data analysis, and interpretation. He demonstrated the role of DEPDC1A in promoting a proliferative phenotype in myeloma cells, and showed that its inhibition induces **cell cycle
Citation
Publication: In PLoS One
Date: April 30, 2013
Type: Journal Article
PDF: Download the PDF
Scientific Contributions
Here are more scientific abstracts authored or co-authored by Alboukadel Kassambara. These contributions span computational biology, bioinformatics, biostatistics, machine learning, and multi-omics, with a focus on immuno-oncology and translational research.
