Collignon A, Kassambara A, Trinh A, Chamourin M, Culley G, Olive M, Jaume C, Benchaaben A, Galon J, Fieschi J. 957 Assessment of the spatial distribution and cell-to-cell interactions into the tumor micro-environment, within and between serial slides stained with Brightplex®, a sequential chromogenic multiplex assay. Journal for ImmunoTherapy of Cancer. 2022. Poster #957, SITC Annual Meeting. View Abstract
Summary of the Study
Accurate characterization of the tumor microenvironment (TME), including immune cell distribution and interactions, is crucial for improving immunotherapy outcomes. This study introduces a spatial analytics tool built around Brightplex® multiplex IHC, digital pathology, and custom R-based analytics.
Key contributions:
Developed spatial analysis features using the
spatstatR package, including:- Proximity metrics
- G-function interaction scores
- Kernel density (heatmap)–based spatial mapping
Integrated spatial analysis across serial slides stained with distinct Brightplex® multiplex panels
Demonstrated proximity of Tregs to CD8+ T cells and macrophages to tumor cells as potential predictors of outcome
Designed to support clinical decision-making and biomarker discovery through integration with the Immunogram framework
In this study, Alboukadel Kassambara contributed to the analysis and software development, building a dedicated R package for spatial analytics. He implemented the bioinformatics workflow, standardized the data pipeline, and integrated it with Brightplex® multiplex staining to enable robust, reproducible spatial quantification of tumor–immune cell interactions.
Citation
Presentation: Poster at SITC 37th Annual Meeting
Date: November 2022
Type: Poster Presentation
PDF: View Abstract
Scientific Contributions
Here are more scientific abstracts authored or co-authored by Alboukadel Kassambara. These contributions span computational biology, bioinformatics, biostatistics, machine learning, and multi-omics, with a focus on immuno-oncology and translational research.
