# Comparing Multiple Means in R

## Friedman Test in R

The Friedman test is a non-parametric alternative to the one-way repeated measures ANOVA test. It extends the Sign test in the situation where there are more than two groups to compare.

Friedman test is used to assess whether there are any statistically significant differences between the distributions of three or more paired groups. It’s recommended when the normality assumptions of the one-way repeated measures ANOVA test is not met or when the dependent variable is measured on an ordinal scale.

In this chapter, you’ll learn how to:

• Compute Friedman test in R
• Perform multiple pairwise-comparison between groups, to identify which pairs of groups are significantly different.
• Determine the effect size of Friedman test using the Kendall’s W.

Contents:

#### Related Book

Practical Statistics in R II - Comparing Groups: Numerical Variables

## Prerequisites

Make sure you have installed the following R packages:

• tidyverse for data manipulation and visualization
• ggpubr for creating easily publication ready plots
• rstatix provides pipe-friendly R functions for easy statistical analyses.

library(tidyverse)
library(ggpubr)
library(rstatix)

## Data preparation

We’ll use the self esteem score dataset measured over three time points. The data is available in the datarium package.

data("selfesteem", package = "datarium")
head(selfesteem, 3)
## # A tibble: 3 x 4
##      id    t1    t2    t3
##   <int> <dbl> <dbl> <dbl>
## 1     1  4.01  5.18  7.11
## 2     2  2.56  6.91  6.31
## 3     3  3.24  4.44  9.78

Gather columns t1, t2 and t3 into long format. Convert id and time variables into factor (or grouping) variables:

selfesteem <- selfesteem %>%
gather(key = "time", value = "score", t1, t2, t3) %>%
convert_as_factor(id, time)
head(selfesteem, 3)
## # A tibble: 3 x 3
##   id    time  score
##   <fct> <fct> <dbl>
## 1 1     t1     4.01
## 2 2     t1     2.56
## 3 3     t1     3.24

## Summary statistics

Compute some summary statistics of the self-esteem score by groups (time):

selfesteem %>%
group_by(time) %>%
get_summary_stats(score, type = "common")
## # A tibble: 3 x 11
##   time  variable     n   min   max median   iqr  mean    sd    se    ci
##   <fct> <chr>    <dbl> <dbl> <dbl>  <dbl> <dbl> <dbl> <dbl> <dbl> <dbl>
## 1 t1    score       10  2.05  4.00   3.21 0.571  3.14 0.552 0.174 0.395
## 2 t2    score       10  3.91  6.91   4.60 0.89   4.93 0.863 0.273 0.617
## 3 t3    score       10  6.31  9.78   7.46 1.74   7.64 1.14  0.361 0.817

## Visualization

Create a box plot and add points corresponding to individual values

ggboxplot(selfesteem, x = "time", y = "score", add = "jitter") ## Computation

We’ll use the pipe-friendly friedman_test() function [rstatix package], a wrapper around the R base function friedman.test().

res.fried <- selfesteem %>% friedman_test(score ~ time |id)
res.fried
## # A tibble: 1 x 6
##   .y.       n statistic    df        p method
## * <chr> <int>     <dbl> <dbl>    <dbl> <chr>
## 1 score    10      18.2     2 0.000112 Friedman test

The self esteem score was statistically significantly different at the different time points during the diet, X2(2) = 18.2, p = 0.0001.

## Effect size

The Kendall’s W can be used as the measure of the Friedman test effect size. It is calculated as follow : W = X2/N(K-1); where W is the Kendall’s W value; X2 is the Friedman test statistic value; N is the sample size. k is the number of measurements per subject (M. T. Tomczak and Tomczak 2014).

The Kendall’s W coefficient assumes the value from 0 (indicating no relationship) to 1 (indicating a perfect relationship).

Kendall’s W uses the Cohen’s interpretation guidelines of 0.1 - < 0.3 (small effect), 0.3 - < 0.5 (moderate effect) and >= 0.5 (large effect). Confidence intervals are calculated by bootstap.

selfesteem %>% friedman_effsize(score ~ time |id)
## # A tibble: 1 x 5
##   .y.       n effsize method    magnitude
## * <chr> <int>   <dbl> <chr>     <ord>
## 1 score    10   0.910 Kendall W large

A large effect size is detected, W = 0.91.

## Multiple pairwise-comparisons

From the output of the Friedman test, we know that there is a significant difference between groups, but we don’t know which pairs of groups are different.

A significant Friedman test can be followed up by pairwise Wilcoxon signed-rank tests for identifying which groups are different.

Note that, the data must be correctly ordered by the blocking variable (id) so that the first observation for time t1 will be paired with the first observation for time t2, and so on.

Pairwise comparisons using paired Wilcoxon signed-rank test. P-values are adjusted using the Bonferroni multiple testing correction method.

# pairwise comparisons
pwc <- selfesteem %>%
wilcox_test(score ~ time, paired = TRUE, p.adjust.method = "bonferroni")
pwc
## # A tibble: 3 x 9
## * <chr> <chr>  <chr>  <int> <int>     <dbl> <dbl> <dbl> <chr>
## 1 score t1     t2        10    10         0 0.002 0.006 **
## 2 score t1     t3        10    10         0 0.002 0.006 **
## 3 score t2     t3        10    10         1 0.004 0.012 *

All the pairwise differences are statistically significant.

Note that, it is also possible to perform pairwise comparisons using Sign Test, which may lack power in detecting differences in paired data sets. However, it is useful because it has few assumptions about the distributions of the data to compare.

Pairwise comparisons using sign test:

pwc2 <- selfesteem %>%
sign_test(score ~ time, p.adjust.method = "bonferroni")
pwc2

## Report

The self-esteem score was statistically significantly different at the different time points using Friedman test, X2(2) = 18.2, p = 0.00011.

Pairwise Wilcoxon signed rank test between groups revealed statistically significant differences in self esteem score between t1 and t2 (p = 0.006); t1 and t3 (0.006); t2 and t3 (0.012).

# Visualization: box plots with p-values
pwc <- pwc %>% add_xy_position(x = "time")
ggboxplot(selfesteem, x = "time", y = "score", add = "point") +
stat_pvalue_manual(pwc, hide.ns = TRUE) +
labs(
subtitle = get_test_label(res.fried,  detailed = TRUE),
caption = get_pwc_label(pwc)
) • Pytchi

Hi! thanks for your article, anyway I am stuck at one point, right when I try to run the friedman_test() function: at this point, I got an error message “not an unreplicated complete block design”
Do you know what could cause such an error?

• Kassambara

Hi, please make sure that your data is correctly formatted as the demo data provide here

• Nick

Hi. When I try to run the Friedman test I get the same error as Pytchi, namely, “not an unreplicated complete block design”. I have formatted my data as in the example with exactly one observation for each combination of grouping factors.

• Nick

I am getting the same error as another used has posted: “not an unreplicated complete block design”. I have made sure my data is formatted as in the example.

• Kassambara

Thank you for your input, appreciated!

• Lu

Thank you for the amazing tutorials!
Unfortunately I am stuck with the visualisation: when entering

I get the following error:
Error: x must be a vector, not a tbl_df/tbl/data.frame/rstatix_test/wilcox_test object.

• Kassambara

• Emilia

Hi, do you know why when I do the box plot with the p-values I get Error in.valide_y_position (y.position, data):
can’t find the y.position variable ‘y.position’ in the data??

• Olaniyi Sonubi

Why is Nemenyi Post-hoc Test not done or is the Wilcoxon signed rank test a substitute to that

• Leander

Hey. Great tutorial!
I am comparing five groups with the Wilcoxon test and have quite many significant differences, so the plot looks very messy.
Is there a way to visualize the pairwise comparison results using the lettering with your approach?

• Laura

Hi, thanks for this helfpul tutorial. It was working perfectly for me until I recently updated my R to version 4.0.5, and reinstalled the packages. Now, when I run the friedmans test, I get the error: Error in UseMethod(“friedman_test”) :
no applicable method for ‘friedman_test’ applied to an object of class “data.frame”. Do you know how to resolve this issue? Many thanks, Laura

#### Course Curriculum

##### Teacher 